Portuguese Mass Spectrometry Network (RNEM)
Core Data
Hosting Legal Entity
Faculdade de Ciências da Universidade de Lisboa
Campo Grande, Faculdade de Ciências da Universidade de Lisboa, Lisboa, PO: 1749-016, Lisboa (Portugal)
Legal Status
University or higher education institution
Av. Rovisco Pais, IPFN-Complexo, Instituto Superior Tecnico, Lisbon, PO: 1049-001, Portugal (Portugal)
Type Of RI
Coordinating Country
Provider Country:
CNC-UC - Centro de Neurociências e Biologia Celular da Universidade de Coimbra (CNC-UC) | Faculdade de Ciências da Universidade de Lisboa | Faculdade de Farmácia da Universidade de Lisboa | INSA-RJ - Instituto Nacional de Saúde Doutor Ricardo Jorge | Instituto Superior Técnico da Universidade de Lisboa | IPATIMUP - Instituto de Patologia e Imunologia Molecular da Universidade do Porto | ITQB NOVA - Instituto de Tecnologia Química e Biológica António Xavier | Universidade da Madeira | Universidade de Aveiro | Universidade de Coimbra
Current Status:
Being upgraded since 2017
Scientific Description
Mission and objectives
The Portuguese Mass Spectrometry Network (Rede Nacional de Espectrometria de Massa,RNEM) is a distributed research infrastructure providing state-of-the-art scientific expertise, advanced technology, training and services in mass spectrometry and proteomics and its applications in medical and life sciences. Established in 2008 under FCT scientific re-equipment program, became integrated in the National Roadmap in 2013. RNEM comprises 10 Nodes that have been working synergistically as a scientific network for almost ten years. IPATIMUP in the North region sits at the largest Health research Hub in the country, I3S and will provide proteomics support. In the Centre region, there are 3 Nodes, FFUC, CNC and Universidade de Aveiro, mainly providing small molecule characterization, omics and quantitative analysis. In the Lisboa region, RNEM comprises 5 Nodes, ITQB/IBET, INSARJ (National Observatory of Health), IST/C2TN, FFUL and FCUL, the former being the coordination Node, providing a vast array of capabilities, from inorganic mass spectrometry, omics, small molecule analysis and structural mass spectrometry. A tenth Node, located in the Madeira Archipelago, is a vantage point for natural product analysis and bioprospection.The main objectives of RNEM are to enable scientific excellence, to support innovation, to foster scientific training and highly qualified employment. It empowers Portugal with an analytical capability that is essential for research in medical and life sciences and the industrial sector (agro, food, pharma, biotech and start-ups). The implementation plan of RNEM comprises four Pillars:1) Excellent Research, 2) Services Supporting Academia and Industry, 3) Marketing, and 4) Training and Formation. By rationalizing mass spectrometry and proteomics resources and competences, RNEM is able to provide competitive, state-of-the art services and technologies that support research and innovation in (bio)chemistry, environment, pharmacology and medicine. RNEM also plays a critical role in national security through its association with government agencies (ASAE and LPC-PJ).RNEM is the sole mass spectrometry infrastructure in the country and the only one of its kind in Europe. It represents Portugal in the main international scientific mass spectrometry and proteomics organizations and the participation of Portugal in Instruct, the European Research Infrastructure on Integrative Structural Biology. Instead of being focused on a single application or technology, like its European counterparts, RNEM grants access to virtually all major mass spectrometry applications, through a single access point at its web portal.

RI Keywords
Proteomics, Systems biology, Mass spectrometry
RI Category
Structural Biology Facilities
Analytical Facilities
Genomic, Transcriptomic, Proteomics and Metabolomics Facilities
Scientific Domain
Biological and Medical Sciences
Chemistry and Material Sciences
ESFRI Domain
Health and Food
Natural Products Analysis and Characterization

Analysis of natural products from marine, agricultural and living organisms. Examples of these are sea sponges, algae, sea urchins as well as cork, olive oil and wine.

Protein Identification

Protein identification by MALDI-TOFTOF and LC-MSMS.

Organic Molecule Analysis and Characterization

Organic molecules originating from synthesis (academic and industrial) including compounds of pharmacological applications.

Post-Translational Protein Modification

Bottom-up and Top-down strategies.

Gas-Phase Ion Chemistry

Gas-phase ion chemistry and kinetics of organic and inorganic molecules, including radioactive species.

Top-Down Protein Analysis

Protein identification and characterization.

Protein Quantification

Isotopic labelling and label free.

Fourier Transform Ion Cyclotron Resonance (FTICR) Mass Spectrometer

With ESI and MALDI capability and multiple MSMS modes, including CAD, IRMPD and ECD; nanoLC and capLC systems.

Matrix-Assisted Laser Desorption/Ionization (MALDI) TOFTOF Spectrometer

Coupled to LC-MALDI.


Four systems, three coupled to HPLC systems.

Linear Ion-Trap

Two instruments, both coupled to HPLC systems.


Two instruments, one is and hybrid system. Both coupled to HPLC systems.

Additional Data
Access Type
Access Mode
Excellence Driven
Access Webpage
Users Definition
User Demographics
European Users - 20.0% in 2017
National Users - 75.0% in 2017
Extra-European Users - 5.0% in 2017
European Users - 22.0% in 2016
National Users - 75.0% in 2016
Extra-European Users - 3.0% in 2016
Type of Users
Academic - 75.0%
Public research organisations - 15.0%
Industry/private companies - 10.0%