Leibniz Association
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Address:
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Germany
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Identification of microorganisms; bacterial genomic DNA.
Deposit in the Open Collection Patent deposit Safe deposit.
Callus and Suspension Cultures Cryopreservation methods.
Plant Virus Diagnosis Plant Virus Disease Clinic.
Speciation of Animal Cell Lines Authentication of Human Cell Lines by DNA Typing Online STR Analysis Mycoplasma Detection Mycoplasma Elimination Virus Testing.
MALDI-TOF MS (matrix assisted laser desorption/ionization time-of-flight mass spectrometry) allows after a simple sample preparation the recording of mass spectra of large biomolecules (mainly ribosomal proteins) which are specific to the microorganism under study. These mass spectrometric fingerprints are used for the reliable identification of bacteria, yeasts and fungi by comparison with reference databases. This method is a promising alternative to microbiological techniques for identification at the species level because it is automated, fast, cost-efficient and suited for highthroughput routine analyses.
The PACBio-sequencer is a device with a high sequencing yield, able to perform sequencing on a single molecule basis without additional amplification steps. In addition it can produce long read lengths of 3,100 bp and 15,000 bp (maximum read length) in the genome regions. The new device (PacBio RS System) was introduced to the market by Pacific Biosciences, a technology company in Menlo Park, California, USA, in April 2011.
Flow Cytometry and Fluorescence Activated Cell Sorting (FACS) represents a fast and reliable method for characterizing and isolating certain cell subpopulations based on fluorescent labeling, and can be applied to a great variety of organisms and cell types, including prokaryotic as well as eukaryotic cells.