The Norwegian Sequencing Centre is a national technology core facility offering sequencing services on the HiSeq X, HiSeq 3000/4000, HiSeq 2500, NextSeq 500 & MiSeq instruments from Illumina, and also the PacBio RS II from Pacific Biosciences.The Norwegian High-Throughput Sequencing Centre (NSC) is a consolidation of the sequencing platforms at the Department of Medical Genetics (AMG) Oslo University Hospital And University Of Oslo (UiO) and Centre for Ecological and Evolutionary Synthesis (CEES), University of Oslo (UiO).The centre was formally established at an opening seminar held on 22 June 2009. The two partners have complementary strengths and research interests. While the CEES node has a particular focus on de novo sequencing and genome assembly, the AMG node specialises in whole-genome resequencing (WGS), amplicon sequencing and epigenetics. Both nodes also have considerable experience and research interests in transcriptomics and metagenomics. The NSC is therefore well positioned to provide the full-range of sequencing services in the wide variety of possible high-throughput sequencing applications.The NSC is funded by Southeastern Regional Health Authorities and the Research Council of Norway (RCN) under the FUGE programme. Additional funding is awarded by the Research Council of Norway (RCN) under the INFRASTRUCTURE programme.