You are here: Home / Infrastructures / Res. Infrastructure
CRG/UPF Proteomics Unit (CRG-PU)
Identification
Hosting Legal Entity
Centre for Genomic Regulation
Address:
Carrer Dr. Aiguader 88, Barcelona, PO: 8003 (Spain)
Location
Dr. Aiguader 88, Center for Genomic Regulation, Barcelona, PO: 08003, Catalunya (Spain)
Structure
Type Of RI
Single-sited
Coordinating Country
Spain
Participating Countries
Spain
Status
Status
Current Status:
Operational since 2001
Scientific Description
The Proteomics Unit is a joint effort of the Center for Genomics Regulation and the Universitat Pompeu Fabra to create an innovative core facility with the mission to offer high-quality proteomics services with strong added-value to the scientific community. The CRG/UPF Proteomics Unit supports basic and translational research projects and it ensures the quality, reproducibility, robustness and traceability of all the offered services. The services offered by the Proteomics Unit are open to all researchers regardless of their institution and the access procedures and service fees are publicly available. Moreover, the Proteomics Unit conducts methodological research as an essential task to keep the unit at the forefront of the proteomics field, and establishes all necessary collaborations to develop new analytical methods, novel mass spectrometric pipelines and new data analysis approaches. Both, development and implementation of new methods and analytical tools constitutes the second essential pillar of the unit to guarantee high-quality services to the users in the mid- and long-term run. Finally community training and dissemination constitutes the third pillar of the CRG Proteomics Unit as a true investment that results in awareness of the current proteomics challenges, improvement of experiment designs, in better project planning, and in realistic expectations, thus leading to better scientific results and higher research quality. The Proteomics Unit of the Center for Genomics Regulation and the University Pompeu Fabra has five high-end mass spectrometers (including a Orbitrap Fusion Lumos, a LTQ-Orbitrap XL, a LTQ-Orbitrap Velos, an QTRAP 5500, and a QTOF 5600), seven HPLC, as well as all the other support infrastructure (wet laboratory, cell culture, ITC, etc) required for providing high-end proteomics services. Moreover, it organizes several training and dissemination activities such as the “Annual Proteomics Symposium” (2009-2017), the “Courses@CRG: Advanced Proteomics Course” (2013-2017), the “EMBO Practical Course on Targeted Proteomics” (2014-2017), and it is a reference Proteomics Unit of the Spanish National Health System as part of the “Plataforma de Recursos Biomoleculares y Bioinformáticos (ProteoRed, Instituto Carlos III)”.

RI Keywords
Phosphopeptides, Peptide, PTM, Phosphorylation, SRM, Targeted proteomics, Proteomics, Mass spectrometry, Post-translational modifications, Proteome, Chromatography, Proteins, Quantification
Classifications
RI Category
Genomic, Transcriptomic, Proteomics and Metabolomics Facilities
Scientific Domain
Biological and Medical Sciences
ESFRI Domain
Health and Food
Services
Protein Identification by nLC-MS/MS
Molecular weight determination of peptides and proteins
Chromatographic Protein and Peptide Separation (Reverse Phase, Strong Cation Exchange, Strong Anion Exchange, ERLIC, Size exclusion)
Protein quantification by label-free strategies of complex mixtures
Identification of Protein post-translational modifications (phosphorylation, glycosilation, methylation, acetylation, ubiquitination)
Protein quantification by Selected Reaction Monitoring
Polyacrylamide Gel Protein Separation (1-Dimension and 2-Dimensions).
Mass spectrometric data analysis and interpretation
Experimental design for mass spectrometric studies
Protein Digestion (FASP, In-solution, In-gel)
Phospho-Peptide enrichment (TiO2, IMAC)
Plasma depletion (IgG+Alb, Top20)
Protein quantitation by isobaric chemical labelling of a complex mixtures (iTRAQ/TMT, Dimethyl)
Protein Identification by 2-Dimension Polyacrylamide Gel Protein Separation
Selected Reaction Monitoring Assay development
Protein quantitation by non-isobaric chemical labelling of a complex mixtures (SILAC, Dimethyl, AQUA Peptides)
Equipment
Mass spectrometer LTQ-Orbitrap XL (Thermo)
High Pressure nano-Liquid Chromatrographic System Agilent 1200 (Agilent)
High Pressure micro-Liquid Chromatrographic System Agilent 1200 (Agilent)
Mass Spectrometer Orbitrap Fusion Lumos (Thermo)
Mass spectrometer LTQ-Orbitrap Velos Pro (Thermo)
High Pressure nano-Liquid Chromatrographic System NanoLC-Ultra Eksigent (Eksigent)
Mass Spectrometer TripleTOF 5600+ (Sciex)
High Pressure nano-Liquid Chromatrographic System Proxeon 1000 (Thermo)
Mass spectrometer Q-Trap 5500 (Sciex)
High Pressure nano-Liquid Chromatrographic System Proxeon 1000 (Thermo)
High Pressure nano-Liquid Chromatrographic System Proxeon 300 (Thermo)
Access
Access Type
Remote, Physical
Access Mode
Excellence Driven
Access Webpage
Users
Users Definition
Individuals
Collaborations
Networks
ProteoRed
Prime-XS
Core for Life (www.coreforlife.eu)
Date of last update: 01/08/2018
Printable version