The Portuguese Mass Spectrometry Network (Rede Nacional de Espectrometria de Massa,RNEM) is a distributed research infrastructure providing state-of-the-art scientific expertise, advanced technology, training and services in mass spectrometry and proteomics and its applications in medical and life sciences. Established in 2008 under FCT scientific re-equipment program, became integrated in the National Roadmap in 2013. RNEM comprises 10 Nodes that have been working synergistically as a scientific network for almost ten years. IPATIMUP in the North region sits at the largest Health research Hub in the country, I3S and will provide proteomics support. In the Centre region, there are 3 Nodes, FFUC, CNC and Universidade de Aveiro, mainly providing small molecule characterization, omics and quantitative analysis. In the Lisboa region, RNEM comprises 5 Nodes, ITQB/IBET, INSARJ (National Observatory of Health), IST/C2TN, FFUL and FCUL, the former being the coordination Node, providing a vast array of capabilities, from inorganic mass spectrometry, omics, small molecule analysis and structural mass spectrometry. A tenth Node, located in the Madeira Archipelago, is a vantage point for natural product analysis and bioprospection.The main objectives of RNEM are to enable scientific excellence, to support innovation, to foster scientific training and highly qualified employment. It empowers Portugal with an analytical capability that is essential for research in medical and life sciences and the industrial sector (agro, food, pharma, biotech and start-ups). The implementation plan of RNEM comprises four Pillars:1) Excellent Research, 2) Services Supporting Academia and Industry, 3) Marketing, and 4) Training and Formation. By rationalizing mass spectrometry and proteomics resources and competences, RNEM is able to provide competitive, state-of-the art services and technologies that support research and innovation in (bio)chemistry, environment, pharmacology and medicine. RNEM also plays a critical role in national security through its association with government agencies (ASAE and LPC-PJ).RNEM is the sole mass spectrometry infrastructure in the country and the only one of its kind in Europe. It represents Portugal in the main international scientific mass spectrometry and proteomics organizations and the participation of Portugal in Instruct, the European Research Infrastructure on Integrative Structural Biology. Instead of being focused on a single application or technology, like its European counterparts, RNEM grants access to virtually all major mass spectrometry applications, through a single access point at its web portal.