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Group for Combinatorial Chemistry, Cell-based sCreening and Chemogenomics (G5C)
Identification
Hosting Legal Entity
French Alternative Energies and Atomic Energy Commission
Address:
Gif-sur-yvette cedex, PO: 91191 (Undefined)
Location
17 rue des Martyrs, CEA DSV, Grenoble, PO: 38 054 (France)
Structure
Type Of RI
Distributed
Coordinating Country
France
Status
Status
Current Status:
Operational since 2001
Timeline
Being upgraded since 2013 to 2013
Scientific Description
G5C (French acronym for "Groupe de Chimie Combinatoire, Criblage Cellulaire et Chemogénomique") is a platform open to the scientific community which develops chemical biology (or chemogenomics) approaches by automated screening of collections of chemical products, characterization of their biological activity and identification of their cellular target. The objective of the G5C platform is to promote the use of chemicals as (i) research tools allowing for deep deciphering of the function of a biological target in physio-pathological processes and (ii) drug candidates for therapeutic purposes.The G5C includes two groups with complementary expertise in biology and chemistry:(1) The CMBA (Screening for BioActive Molecules platform at iRTSV, CEA-Grenoble) offers the possibility to perform large automated screens in either in vitro or in cellulo assays. Two automated microscopes with different specificities are used for the screening of either selected hits or small libraries using High Content Analysis/Screening methods.(2) The CCCHD (Centre of Combinatorial Chemistry and High throughput screening, at iBiTec-S, CEA-Saclay) allows for screening with radioactive compounds. It can guide the choice of chemical analogues via either private companies or in-house chemical synthesis.Access to G5C's facilities and compounds collections can be had either in collaboration mode or through provision of service.

RI Keywords
Hts, Cell-based assays, High content screening, HTRF, Statistical data analysis, Fluorescence, Automated screening, Pharmacomodulation, High content analysis, Chemogenomics, Radioactivity, Polarization of fluorescence, Enzymatic assays, Luminescence, Hcs, Protein-protein interaction assays, Pharmacology, Chemical synthesis, Hca, Compounds libraries
Classifications
RI Category
Chemical Libraries and Screening Facilities
Scientific Domain
Chemistry and Material Sciences
Services
Access to automated HTS and HCS/HCA facilities and expertise to identify new chemical, bio-active research tools or potential therapeutic leads inside compounds libraries

The services offered can be summarised as follows: • Analysis of the project and evaluation of its feasibility • Advice and assistance at the platform for miniaturisation and statistical analysis of assay robustness • Access to and assistance in the choice of collections of chemical compounds • Robot programming and optimisation of the automated assay • Screening of all or part of the collections of chemical compounds in 96-or 384-well plates • Data analysis, selection of hits, purchase of chemically closely related molecules • Hit-picking and validation screening • Transfer of results (minimally as raw data associated with the compound structures from the library) • Synthesis of small focused libraries • SAR-based optimization of hits using a fragment-based approach • Design of new chemical reactions for synthesis of second-generation libraries • Provision of molecular tools to trap these targets.

Equipment
Integrated facility for HTS and cell-based phenotypic HCS/HCA screening

A dedicated room for the robotic platform containing 1) a Tecan's Evo liquid handling work station with a AirLiHA 8-channel pipetting head and a MCA 96-channel pipetting head, both using disposable tips; a robotic arm for microplates and tip boxes management, a Tecan's Hydrospeed 96/384-well microplate washer fully adapted for washing weakly adherent cells, a Liconic's StoreX automated cell incubator, a plate shaker, a carousel for storing tip boxes and microplates, two barcode readers and Tecan's Infinite M100 multimode, monochromator-based plate reader for quantifying signal in fluorescence, chemiluminescence and absorbance; 2) an imaging room including automated microscopes and dedicated computers for HCA/HCS activity; 3) a cell culture room to prepare large amounts of cultured cells; 4) a molecular biology laboratory for setting up miniaturized assays, 5) Several alarm-connected -20°C freezers for chemical libraries storage, 6) for chemical synthesis, a microwave synthesis device, two LC/MS, a 400 MHz NMR spectrometer with an automatic sampler. The HTS workstation has been recently placed into a custom-designed Class II Biological Safety Cabinet in order to perform compounds screening using Class II biological agents (bacteria, viruses, cells...).

Collaborations
Networks
GDR 3056 "ChemBioScreen"
EU-OPENSCREEN
Date of last update: 27/04/2015
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