The services offered can be summarised as follows: • Analysis of the project and evaluation of its feasibility • Advice and assistance at the platform for miniaturisation and statistical analysis of assay robustness • Access to and assistance in the choice of collections of chemical compounds • Robot programming and optimisation of the automated assay • Screening of all or part of the collections of chemical compounds in 96-or 384-well plates • Data analysis, selection of hits, purchase of chemically closely related molecules • Hit-picking and validation screening • Transfer of results (minimally as raw data associated with the compound structures from the library) • Synthesis of small focused libraries • SAR-based optimization of hits using a fragment-based approach • Design of new chemical reactions for synthesis of second-generation libraries • Provision of molecular tools to trap these targets.

A dedicated room for the robotic platform containing 1) a Tecan's Evo liquid handling work station with a AirLiHA 8-channel pipetting head and a MCA 96-channel pipetting head, both using disposable tips; a robotic arm for microplates and tip boxes management, a Tecan's Hydrospeed 96/384-well microplate washer fully adapted for washing weakly adherent cells, a Liconic's StoreX automated cell incubator, a plate shaker, a carousel for storing tip boxes and microplates, two barcode readers and Tecan's Infinite M100 multimode, monochromator-based plate reader for quantifying signal in fluorescence, chemiluminescence and absorbance; 2) an imaging room including automated microscopes and dedicated computers for HCA/HCS activity; 3) a cell culture room to prepare large amounts of cultured cells; 4) a molecular biology laboratory for setting up miniaturized assays, 5) Several alarm-connected -20°C freezers for chemical libraries storage, 6) for chemical synthesis, a microwave synthesis device, two LC/MS, a 400 MHz NMR spectrometer with an automatic sampler. The HTS workstation has been recently placed into a custom-designed Class II Biological Safety Cabinet in order to perform compounds screening using Class II biological agents (bacteria, viruses, cells...).