Bijvoet Center for Biomolecular Research
Heidelberglaan 8, Utrecht, PO: 3584 CS (Netherlands)
Genomic, Transcriptomic, Proteomics and Metabolomics Facilities
Biological and Medical Sciences
The solution state NMR infrastructure at the Bijvoet Center of Utrecht University group consists of a 950 MHz, a 900 MHz, a 750 MHz, two 600 MHz and a 500 MHz NMR spectrometer completely up to date with respect to current and future needs of high resolution NMR. The 950, 900 and one of the 600 MHz spectrometers are equipped with cryprobe systems for enhanced sensitivity. The 500 MHz spectrometer can be equipped with a laser system for light-related experiments.
Developed by the NMR spectroscopy group at the Bijvoet Center of Utrecht University, several web portals provide access to powerful software packages ported. A wide range of structural biology related software packages are available covering the many stages in NMR structure calculation, molecular dynamics simulations and structure modelling.
The National Single Crystal X-ray Facility at the Bijvoet Center of Utrecht University offers crystal structure determinations to synthetic chemists at universities, institutes and companies in The Netherlands. With experienced staff, state-of-the-art equipment and advanced software we are able to handle a large variation of chemical compounds.
The Biomolecular Mass Spectrometry and Proteomics group at Utrecht University provides an expert centre for state of the art proteomics analysis. Innovative mass spectrometric methods are developed and implemented for the efficient and detailed characterization of biomolecules in their relation to their biological function. The scientific focus ranges from single protein identifications to the analysis of complex, whole cell lysate, samples and determination of posttranslational modifications. The group is part of the Netherlands Proteomics Centre (NPC) and the Bijvoet Center for Biomolecular Research.
The EM facility at the Bijvoet Center of Utrecht University is devoted to the development and application of technologies for specimen preparation, electron microscopy data collection, as well as image analysis for cell biology and material sciences applications. The infrastructure includes several Transmission Electron Microscopes (TEM), Scanning Electron Microscopes (SEM) with a full range of attachments such as STEM, EDX and energy-filtering. The facility houses the equipment to prepare specimen with preservation of ultrastructures (including cryo-fixation and freeze-substitution of samples), immunolabeling, serial-sectioning and high-resolution electron tomography to study three dimensional structure of cells, tissues and other materials.
Native protein mass spectrometry (part of the Biomolecular Mass Spectrometry and Proteomics group, Bijvoet Center, Utrecht University) provides a rapid and sensitive means for assessing the quaternary organisation of proteins. It is widely applicable, including antibodies and assemblies as large as viruses, containing intrinsically disordered regions, associated with membranes, or populating a range of oligomeric states. The state-of-the-art enables the determination of inter-subunit connectivity, protein shape and topology, and equilibrium dynamics.
Tecnai 12 Transmission Electron Microscope.
Nova 600 NanoLab Scanning Electron Microscope.
Tecnai 20 Transmission Electron Microscope with STEM, BF and DF.
The facility hosts about 50000 CPU-cores and 200 TB of storage for distributed computing purposes related to structural biology.
Tecnai 10 Transmission Electron Microscope.
Q-Exactive Mass Spectrometer.
Tecnai 20 Transmission Electron MicroscopeFEG with STEM, HAADF, EDX, DF, BF and SE.
XL30S FEG Scanning Electron Microscope.