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Bijvoet Center for Biomolecular Research (Bijvoet Center)
Identification
Hosting Legal Entity
Bijvoet Center for Biomolecular Research
Utrecht University
Address:
Heidelberglaan 8, Utrecht, PO: 3584 CS (Netherlands)
Location
Padualaan 8, Nicolaas Bloembergen Building, Utrecht, PO: 3584 CH (Netherlands)
Structure
Type Of RI
Single-sited
Coordinating Country
Netherlands
Status
Status
Current Status:
Operational since 1988
Scientific Description
The Bijvoet Center for Biomolecular Research at Utrecht University in the Netherlands was founded in 1988 and aims to gain insight into the relation, at the molecular level, between the structure and the function, and activity of biomolecules that are involved in recognition, interaction, and regulatory processes. To achieve this goal, the Bijvoet Center for Biomolecular Research maintains an advanced infrastructure and provides high-quality research training programs for young scientists.The Bijvoet Center coordinates the EU funded infrastructure projects PRIME-XS (proteomics) and WeNMR (computational structural biology), participates in the infrastructure projects Bio-NMR and EAST-NMR (biomolecular NMR) and acts as a Centre in the ESFRI project Instruct for integrated structural biology.

RI Keywords
Structural biology, Biochemistry, Mass spectrometry, Proteins, Biomolecular modelling, Electron microscopy, Proteomics, Protein assembly, NMR, Lipids, X-ray crystallography
Classifications
RI Category
Genomic, Transcriptomic, Proteomics and Metabolomics Facilities
Structural Biology Facilities
Bio-informatics Facilities
Scientific Domain
Chemistry and Material Sciences
Biological and Medical Sciences
ESFRI Domain
Health and Food
Services
Native protein complex mass spectrometry

Native protein mass spectrometry (part of the Biomolecular Mass Spectrometry and Proteomics group, Bijvoet Center, Utrecht University) provides a rapid and sensitive means for assessing the quaternary organisation of proteins. It is widely applicable, including antibodies and assemblies as large as viruses, containing intrinsically disordered regions, associated with membranes, or populating a range of oligomeric states. The state-of-the-art enables the determination of inter-subunit connectivity, protein shape and topology, and equilibrium dynamics.

Electron Microscopy

The EM facility at the Bijvoet Center of Utrecht University is devoted to the development and application of technologies for specimen preparation, electron microscopy data collection, as well as image analysis for cell biology and material sciences applications. The infrastructure includes several Transmission Electron Microscopes (TEM), Scanning Electron Microscopes (SEM) with a full range of attachments such as STEM, EDX and energy-filtering. The facility houses the equipment to prepare specimen with preservation of ultrastructures (including cryo-fixation and freeze-substitution of samples), immunolabeling, serial-sectioning and high-resolution electron tomography to study three dimensional structure of cells, tissues and other materials.

Small molecular X-ray structure determination

The National Single Crystal X-ray Facility at the Bijvoet Center of Utrecht University offers crystal structure determinations to synthetic chemists at universities, institutes and companies in The Netherlands. With experienced staff, state-of-the-art equipment and advanced software we are able to handle a large variation of chemical compounds.

Molecular modeling / docking simulation

Developed by the NMR spectroscopy group at the Bijvoet Center of Utrecht University, several web portals provide access to powerful software packages ported. A wide range of structural biology related software packages are available covering the many stages in NMR structure calculation, molecular dynamics simulations and structure modelling.

High-throughput, large scale, proteomics analysis

The Biomolecular Mass Spectrometry and Proteomics group at Utrecht University provides an expert centre for state of the art proteomics analysis. Innovative mass spectrometric methods are developed and implemented for the efficient and detailed characterization of biomolecules in their relation to their biological function. The scientific focus ranges from single protein identifications to the analysis of complex, whole cell lysate, samples and determination of posttranslational modifications. The group is part of the Netherlands Proteomics Centre (NPC) and the Bijvoet Center for Biomolecular Research.

Solution state NMR / protein structure determination

The solution state NMR infrastructure at the Bijvoet Center of Utrecht University group consists of a 950 MHz, a 900 MHz, a 750 MHz, two 600 MHz and a 500 MHz NMR spectrometer completely up to date with respect to current and future needs of high resolution NMR. The 950, 900 and one of the 600 MHz spectrometers are equipped with cryprobe systems for enhanced sensitivity. The 500 MHz spectrometer can be equipped with a laser system for light-related experiments.

Equipment
Triple Quadrupole Mass Spectrometer
700 MHz solid state NMR (Bruker Avance III)
Tecnai 20 with STEM, BF and DF

Tecnai 20 Transmission Electron Microscope with STEM, BF and DF.

Tecnai 10

Tecnai 10 Transmission Electron Microscope.

Nova 600 NanoLab

Nova 600 NanoLab Scanning Electron Microscope.

Bruker Kappa Apex II diffractometer
Q-ToF Mass Spectrometer
Q-Exactive Mass Spectrometer

Q-Exactive Mass Spectrometer.

500 MHz solid state NMR (Bruker Avance III)
XL30S FEG

XL30S FEG Scanning Electron Microscope.

Orbitrap Mass Spectrometer
800 MHz solid state NMR (Bruker Avance III) with DNP
500 MHz NMR (Bruker AvanceII) with laser system
Ion Trap Mass Spectrometer
750 MHz NMR (Bruker AvanceII)
600 MHz NMR (Bruker AvanceIII)
MALDI Mass Spectrometer
Tecnai 20 FEG with STEM, HAADF, EDX, DF, BF and SE

Tecnai 20 Transmission Electron MicroscopeFEG with STEM, HAADF, EDX, DF, BF and SE.

400 MHz solid state NMR (Bruker Avance III) with DNP
Bruker Proteum diffractometer
Tecnai 12

Tecnai 12 Transmission Electron Microscope.

500 MHz NMR (Bruker AvanceDRX)
Local clusters and European Grid Initiative distributed computing infrastructure

The facility hosts about 50000 CPU-cores and 200 TB of storage for distributed computing purposes related to structural biology.

600 MHz NMR (Bruker AvanceII) with TCI cryoprobe
Collaborations
Networks
DTL
INSTRUCT
INSTRUCT-NL
EAST-NMR
Bio-NMR
Ti-COAST
PRIME-XS
WeNMR
European Grid Infrastructure (EGI)
Date of last update: 11/05/2017
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