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Core Facility Cellular Analysis (CA)
Identification
Hosting Legal Entity
University of Natural Resources and Life Sciences, Vienna
Address:
Austria
Location
Muthgasse 18, TZM, Wien, PO: 1190 (Austria)
Structure
Type Of RI
Single-sited
Coordinating Country
Austria
Status
Status
Current Status:
Operational since 2010
Scientific Description
EQ offers access to state-of-the-art scientific equipment to research organizations, companies and universities on a pay-by-use basis. It also offers a full-service solution for the operation of all scientific equipment in our portfolio. Equipment services are particularly well-suited for projects in the areas of:- Chemistry- Biochemistry- Genetics- Cell biology- Molecular biology- Biotechnology- Nanobiotechnology- Bioinformatics- Food sciences- Enzyme technology- Water and sewageThe combination of mass spectrometric instrumentation - to apply highly sensitive methods for proteomics and metabolomics analysis with smallest amounts of sample material - with a cell sorter, allows the selection of defined subpopulations for industrial use.

RI Keywords
Proteomics, Metabolomics, Cell sorting
Classifications
RI Category
Analytical Facilities
Scientific Domain
Biological and Medical Sciences
ESFRI Domain
Health and Food
Services
Access

EQ offers you the access to well-equipped scientific Core Facilities, provides optional scientific services and establishes strong collaborations with research partners from the Vienna Institute of Bio¬Technology. Therefore, additional benefits from the access to a cost-effective infrastructure and the on-site scientific expertise are achieved. In addition to provide technical assistance for the instrumentation, scientific services such as sample analysis, consultancy, planning applied research concepts or feasibility studies which are related to the equipment in the Core Facility can be carried out. Furthermore, EQ offers personalized user training for the available instrumentation.

Equipment
LC-MS/MS and GC MS/MS

Highly sensitive mass spectrometer for quantitative analysis of small molecules; metabolomics. The Thermo Vantage triple quadrupole LC-MS/MS system consists of a 1000 bar Accela HPLC System for ultra-fast separations, electrospray or atmospheric pressure chemical ionization ion source options and a fast scanning MS/MS mass spectrometer with enhanced resolution.

Nano-LC-tandem-MS

Highly selective mass spectrometer for proteomics and glycoproteomics applications. The instrument will consist of a Dionex RSLC (rapid separation liquid chromatography) unit that can operate gradient elution of nano-columns with sub-2 μm particles in order to achieve short analysis times or highest chromatographic resolution. The detector is a Bruker Maxis 4G, which is among the most sensitive mass spectrometers on the market and the Q-TOF MS with the highest currently achievable resolution (60.000 FWHM) and mass accuracy (sub ppm). The instrument shall also be equipped with an ETD source allowing an alternative fragmentation technique e.g. for peptides with post-translational modifications.

High Speed Cell Sorter

The MoFlo Astrios is a high speed cell sorter with improved sensitivity. Equipped with five lasers the instrument can analyse up to 13 size and fluorescence parameters. Isolation of cells with defined properties for subsequent culture or further analysis is possible both in bulk and into microtiter plates and will allow in depth studies of cellular phenotypes. Biosafety aersol containment allows sterile sorting of up to six subpopulations for cell line development.

Date of last update: 17/03/2017
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