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UCD Conway Institute - Core Technologies (Conway)
Identification
Hosting Legal Entity
University College Dublin
Location
University College Dublin, Belfield, UCD Conway Institute of Biomolecular and Biomedical Research, Dublin, PO: Dublin4, Leinster (Ireland)
Structure
Type Of RI
Single-sited
Coordinating Country
Ireland
Status
Status
Current Status:
Operational since 2003
Scientific Description
UCD Conway Institute of Biomolecular and Biomedical Research is a multidisciplinary centre for research funded by an initiative of the Irish Higher Education Authority, the Programme for Research in Third Level Institutions (PRTLI). Located on the 300-acre Belfield campus of University College Dublin (UCD), the largest university in Ireland, UCD Conway Institute brings together over 550 research staff from all over the University and its associated teaching hospitals. UCD Conway Institute is an interdisciplinary research centre exploring fundamental mechanisms of chronic disease for novel diagnostic and therapeutic solutions.UCD Conway Institute is a a global leader in biomedical research and through partnerships translates this knowledge to benefit patients.Research at UCD Conway focuses on the molecular pathogenic mechanisms that underlie major chronic diseases: diabetes, vascular diseases, cancer and neurodegeneration. The approach addresses important biomedical questions combining human potential and innovative technologies to develop creative solutions for exploring, understanding, and developing new treatments for human diseases. Through interdisciplinary programmes in the areas below, the close collaboration of scientists with clinicians and industry partners underpins the translational nature of the centre's research.

RI Keywords
Translational medicine, Neuroscience, Cytometry, Toxicology, Tissue engineering, Biopharmaceutical production, Transcriptomics, MicroRNA, Cancer, Flow cytometry, Proteomics, Diabetes, Chemical synthesis, Imaging, Systems biology, Biotechnology, Biological chemistry, Bioinformatics, Stem cell biology, Functional genomics, Pharmacokinetics, Ocular disease, Oncology, Vascular biology, Medicine, Molecular biology
Classifications
RI Category
Genomic, Transcriptomic, Proteomics and Metabolomics Facilities
Systems Biology/Computational Biology Facilities
Bio-informatics Facilities
Scientific Domain
Biological and Medical Sciences
ESFRI Domain
Health and Food
Services
Imaging Core

The UCD Conway Imaging Core where the focus is on providing contemporary imaging technologies and know-how to researchers. The consolidated instrumentation suite spans all aspects of light and electron microscopy as well as sample preparation and image processing and analysis facilities. The Core team use their technical expertise to assist in the widest possible range of applications for microscopy, in advancing the development of new applications and indeed new imaging technologies.

Flow Cytometry Core

The UCD Conway Flow Cytometry Core offers access to a good range of flow cytometers, cell sorter and multiplexing instrumentation. We assist with the project discussion, pannel design, analysis and reanalysis of the data and publication of the results. We offer customised and personalised training courses.

Genomics Core

The Genomics Core has a range of facilities for next-generation sequencing, real-time PCR and microarray analysis as well as offering custom bioinformatics analysis. The facility provides genomics solutions to academic and commercial customers in a customisable range of services for each stage of the research pathway, from experimental design and strategy to final publication.

Proteomics Core

The UCD Conway Proteomics Core service is capable of covering all aspects of modern proteomic science including quantitative proteomics, protein post-translational modifications and protein-protein interactions. We offer the dedicated strategic support of our team, both on the analytical and bioinformatics side, to enable our research and commercial partners to take full advantage of their results.

Equipment
Multiplexing

Luminex xMAP200.

Affymetrix GeneChip

GeneChip 3000 7G scanner Automated fluidics 450 station Hybridisation oven GeneChip workstation with AGCC Software Agilent bioanalyser Nanodrop spectrophotometer.

Light Microscopy

Transmission light microscopy Fluorescent and epi fluorescent microscopy Laser confocal (single pinhole) Spinning disc confocal Fluorescence resonance energy transfer (FRET) Fluorescence-lifetime imaging microscopy (FLIM) Fluorescence recovery after photobleaching (FRAP) Photoactivation Total internal reflection fluorescence (TIRF) FRET/FLIM TIRF confocal microscope Reflected light microscopy.

Flow Cytometry

Beckman Coulter FC500 (blue laser) Beckman Coulter Cyan ADP (blue, red, violet lasers) Beckman Coulter Cyan ADP modified (blue, red, yellow-green laser) BD Accuri C6 (2x).

Bioinformatics

mRNA seq ChIP seq Prokaryotic genome sequencing Affymetrix gene expression arrays.

NanoSight

NanoSight NS300.

Mass Spectrometry Resource

Thermo Fisher Q-ExactiveThermo Fisher Orbitrap Thermo Fisher LTQ ion trap Agilent 6520 Quadrupole-ToF Agilent 6460 Triple Quadrupole ABI 4800 Ultra MALDI/ToF-ToF with CovalX HM2 high-mass range detector Bruker HCTUltra II ETD ion trap.

Protein Separations Laboratory

Protein separation by 2-D gel electrophoresis (2-DE): IPGphorII and IPGphor3; Ettan Dalt 6 and 12; BioRad Protean Plus Dodeca cell Imagers (both visible and fluorescent):Typhoon 9410 variable mode imager (for 2D-DIGE); BioRad GS800 Image analysis software: Progenesis SameSpots v4;DeCyder v6.

Illumina Sequencing Platform

RNA-Seq Digital gene expression Whole genome and candidate region sequencing, DNA-protein interaction profiling and Small RNA identification.

Digital Pathology

ScanScope XT high throughput scanning system (Aperio Technologies) ImageScope image viewer Tissue microarray segmentation tool TMALab Analysis toolbox.

Histological Sample Preparation

Tissue Tek VIV E 300processor Leica EG1150H Automatic stainer Leica XL Automatic coverslipper LeicaCV5030 Labvision pre-treatment (PT) module Multiheaded microscope.

Cell Sorting

FACSAria IIIu (blue, red, violet and yellow green laser) with CPU platform for single cell sorting in well plates.

Real-Time PCR

Two ABI 7900HT Sequence Detection Systems are in operation in this facility comprising: Primer Express Software v2.0 (primer and probe assay design) Interchangeable thermal cycling blocks (Fast and Standard) Laser and CCD camera detector (fluorescence induction and data collection) Sequence Detection Software v2.4 (instrument operation and data analysis).

Transmission and Scanning Electron Microscopy (TEM & SEM)

Three transmission electron microscopes (TEM) One scanning electron micropscope (SEM) Three ultramicrotomes, including one suitable for cryo-ultramicrotomy Critical point dryer.

Date of last update: 23/04/2019
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