We synthesize high quality synthetic DNA (oligos, primers, probes from 10 bases to 90 bases long) with an MWG synthesizer, which can provide 24 different DNA sequences (oligos) per synthesis. The provided oligos, purified, quantified and lyophilized, are sent within 3 working days to the clients. For information and orders, please contact: Mrs. EFTERPI KARPELI – Lab responsible

The FF focuses on controlled production of biomass (mainly microbial) over a wide range of controlled conditions (culture media, temperature, pH, aeration, stirring, etc). The FF houses all equipment needed for the maintenance, growth, collection and storage of microorganisms (bacteria, fungi, etc). Cell mass is produced according to strict quality criteria and is accompanied with Specifications and Technical data sheet that includes all relevant information on growth conditions. What we offer: Optimization of your protein expression or biomass growth and upscale to our bioreactors. You just bring us a plate with your desired bacterial strain and we deliver you cell pellet (from 20lt or 30lt culture).

Large scale (mg to gram) protein purification of tagged or untagged proteins. Our team is highly-experienced in downstream processing steps and can help you optimize your protein purification scheme. A series of different types of chromatography can be tested to your protein (size exclusion, Ion exchange, affinity, hydrophobic interaction). The PFF’s, FPLC equipments are capable of up to 100 ml/min flow rates.

Raw data in BAM fastq format are delivered via a secure FTP site or are shipped on a portable hard drive depending upon the size of the dataset Data Analysis includes: • Base calling and demultiplexing • Quality-adapter trimmed reads • De novo/reference mapping using platform specific, open source or commercial software (CLC Genomics) • On-target and coverage statistics • Variant summary report including variant coordinates, base pair changes, dbSNP and coding region information Further bioinformatics analysis is also possible, depending on your experiment, in collaboration with our recently constituted Bioinformatics group.

The Core Facility is a full service facility that provides expertise in experimental handling for the entire process of sequencing, including library construction, qualification of sequencing libraries by qPCR, and sequence analysis on an Illumina Nextseq500 or Ion Torrent Proton or PGM instruments. Services include: • Whole genome sequencing • Targeted region sequencing • Whole exome sequencing • RNA-Seq • ChIP-Seq