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CERM-CIRMMP NMR Infrastructure (CERM-CIRMMP NMR)
Identification
Hosting Legal Entity
Magnetic Resonance Center/Interuniversity Consortium of Magnetic Resonance Metalloproteins
Location
Via Sacconi 6 Sesto Fiorentino, CERM, Florence, PO: 50019 (Italy)
Structure
Type Of RI
Single-sited
Coordinating Country
Italy
Status
Status
Current Status:
Operational since 1994
Scientific Description
The CERM/CIRMMP infrastructure provides a unique environment for research in the field of bio-nuclear magnetic resonance spectroscopy, with an emphasis on structural biology. The Mission of CERM can be summarized in three main points: (i) identification of proteins starting from genome browsing through a bioinformatic approach; (ii) biophysical characterization and structure determination; (iii) design and development of drugs.CERM, Centre for Magnetic Resonance, is a scientific institution for research and higher education at the University of Florence, and a national laboratory of the Interuniversity Consortium for Magnetic Resonance of MetalloProteins CIRMMP. CERM-CIRMMP is a core centre of the ESFRI Instruct-ERIC for integrated structural biology, a node of the We-NMR electronic infrastructure for computational structural biology, and a partner of several EU funded H2020 projects.CERM-CIRMMP provides: national access to NMR instrumentation for private and public research institutions; transnational European access to the NMR Infrastructure which is offered in the frame of the ESFRI Instruct, and through the EC-funded iNEXT project; transnational access to non-European users, managed directly by the CERM-CIRMMP infrastructure.

RI Keywords
Information technologies, Metabolomics, Nuclear magnetic resonance, NMR, Structural biology, Metalloenzimes, Drug design, Metalloproteins
Classifications
RI Category
Distributed Computing Facilities
Genomic, Transcriptomic, Proteomics and Metabolomics Facilities
Bio-informatics Facilities
Collections of Biological Resources (e.g. Microorganisms, Biobanks and Seed Banks)
Systems Biology/Computational Biology Facilities
Structural Biology Facilities
Analytical Facilities
Scientific Domain
Chemistry and Material Sciences
Biological and Medical Sciences
ESFRI Domain
Health and Food
Services
Sample Storage and Retrieval

Access to da Vinci European BioBank for biological (both human and non-human) sample storage and retrieval.

Access to High Field Solution NMR Spectrometers

Spectrometers operating at field strengths ranging from 400 to 950 MHz and from 0.01 to 50 MHz. The high field instruments are equipped with cryoprobes and/or with a variety of special probes which make them unique for the structural and dynamical characterization of high molecular weight proteins, paramagnetic systems and unfolded or partially unfolded states. In-cell NMR can be used to follow protein folding and processes that are at the basis of mitochondrial function.

Access to Solid State NMR Spectrometers

A 700 MHz WB and a 850 MHz WB instruments, and a narrow bore operating at 600 MHz. These instruments are ideal for the atomic level characterization of insoluble material like large protein aggregates and fibrils. At CERM-CIRMMP an innovative approach has been developed, to study soluble macromolecules, aggregates or prefibrillar oligomers by sedimenting them directly in the NMR rotor by means of exploiting its centrifugal force.

Equipment
700 MHz NMR Spectrometer

Three-channel and 2H decoupling instrument, with a TCI cryoprobe and automatic sample changer.

500 MHz High Field Solution NMR Spectrometer

Three-channel instrument equipped with TCI cryoprobe, TXI RT, TBO RT and BBI RT probes.

600 MHz High Field Solution NMR Spectrometer

Equipped with HR-MAS accessories and a number of BB probes which cover a frequency range from 600 to 20 MHz.

Multi Angle/Dynamic Light Scattering (DLS) Instrument

For measuring batch samples or in-flow samples (FPLC coupling).

700 MHz NMR Spectrometer

Four-channel instrument, equipped with a TXO cryoprobe for 13C direct detection with 1H, 2H, 13C and 15N decoupling.

Fast Field Cycling Relaxometer

High sensitivity Stelar relaxometer for the measurement of relaxation rates from 0.01 to 45 MHz proton Larmor frequency.

Isothermal Calorimetry (ITC) Device

To study the thermodynamics of protein-ligand of protein-protein interactions.

950 MHz High Field Solution NMR Spectrometer

Three-channel and 2H decoupling instrument, equipped with a TCI cryoprobe.

400 MHz High Field Solution NMR Spectrometer

Two-channel instrument equipped with BBO RT, BBI RT, 1H selective high power probes.

850 MHz Solid State Wide Bore NMR Spectrometer

Equipped with MAS accessories for solid-state studies, with 0.7 mm, 1.3 mm and 3.2 mm triple resonance probes.

X-Band and W-Band EPR Spectrometers

9.43 GHz (X-Band, ElexSys E 500) and 35 GHz (W-Band, ElexSys E 580E), with low temperature ancillary equipment.

900 MHz High Field Solution NMR Spectrometer

Three-channel and 2H decoupling instrument, equipped with a TCI cryoprobe and TXI RT probe.

700 MHz Solid State Wide Bore NMR Spectrometer

Equipped with MAS accessories for solid-state studies, with 3.2 mm and 4 mm double and triple resonance probes.

Bioinformatics and Computational Structural Biology Tools

Integrated databases and software for genome browsing, metal binding analysis, structure calculation with/without paramagnetic constraints, sequence validation, domain organization, evolution, protein complex analysis; programs for NMR data processing and structural calculations is also provided via web, and through the Grid computing platforms.

Surface Plasmon Resonance Instrument

For studying protein-ligand or protein-protein interactions.

800 MHz NMR Spectrometer

Four-channel instrument, equipped with a TXI cryoprobe, a QXI RT probe and a high-power probe for the study of fast-relaxing systems and MAS accessories for solid state studies, with 1.3 mm, 1.3 mm and 3.2 mm triple resonance probes.

Circular Dichroism Device

To study the secondary structure of proteins.

600 MHz NMR Spectrometer

Three-channel and 2H decoupling instrument, equipped with a TCI 1H/13C/31P cryoprobe, as well as an automatic sample changer.

Collaborations
Networks
INSTRUCT
WeNMR
BBMRI-ERIC
European Grid Infrastructure (EGI)
iNEXT
Date of last update: 05/07/2019
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