Creation, Breeding, Phenotyping, Distribution anhiving of Vertebrate Mod Arcdels
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Repositories
Animal facilities
Biomedical Imaging Facilities
Husbandry and breeding of several organisms according the center: mouse, rat, other rodent, non human primate, xenope, zebra-fish.
Distribution of wild-type organisms, modified strains, embryos, eggs (fish, xenope), biological material to users.
CELPHEDIA partners offer guidance in the choice of the organism model and a wilde range of courses and workshops for the use of the model.
Distribution of genetically modified mouse strains.
Monitoring the health status of a mouse line (quarantine, decontamination, health/sanitary control), mouse husbandry, mouse breeding, genetic background fixation, genotyping, generation of cohorts, genetic quality control of a mouse model (validation of genetic modification, level of gene expression and tissue expression).
Clinical phenotyping in vivo in the field of anatomic-pathology, behavior, cognition and sensory systems, cardiovascular system, neuromuscular system, nutrition and metabolism, renal function, pulmonary,embryology and reproduction, immunology, inflammation, allergy, infectiology, oncology, clinical chemistry and hematology), in vivo imaging (photonic, 2D and 3D radiologic, isotopic imaging), in vitro and in situ immunophenotyping ( Innate and adaptive immune sytem under normal or confined condition),.
Embryos cryopreservation, sperm cryoconservation, organs, biological material (RNA, DNA, proteins…).
Creation of genetically modified mouse models by transgenesis and targeted mutagenesis, production of ES cells, screening and validation of recombinated ES cells.
Genetic engineering: creation of new transgenic organism models.
Housing and breeding rooms for mouse and rat under various health status (SPF, SOPF, biosafety containment from A2 to A3), in various devices (opened cages, ventilated racks) and with controlled environnement (temperature, light, photoperiod, food quantity).
Closed circuit aquarium (xenope, fish).
Genetically modified strains creation: DNA and ES cells microinjection; microinjection devices for ovocytes; robotic (Tecan) workstations for DNA; microfluidics electrophoresis robots (Caliper); current molecular biology devices (PCR, qPCR, blots, etc...).
Facs analysers; cell sorter; cell separator; Luminex; mass cytometer; polychromatic flow cytometry devices; BSL3 live imaging (confocal and biphotonic imaging).
For non-invasive exploration by molecular imaging, isotopic imaging, and radiology imaging: bioluminiscence, fluorescence, scintigraphy, positron emission tomography, and tomography opto-acoustic equipment.
In vivo model development (mouse) tools; animal testing training (mouse, pig, rabbit); stereotaxy; xenografs; BrainSight type neuro-navigation device.
Material for functional exploration (HPLC, OLYMPUS AU-400 automated laboratory work station, luminex, facs analyser, metabolic cages, comprehensive indirect calorimetry system, exercise treadmill, NMR body composition, echocardiographs, blood pressure measurment device, plethysmograph, telemetry devices); material for testing cognitive functions and behavior (openfield, Y- maze, Morris water-maze, slit lamp imaging, ERG, clic box); histopathology platform.
Conventional housing; A2 containment housing; external dedicated areas.
For non-invasive exploration through: molecular, microscopic, mesoscopic, macroscopic imaging tools.