Creation, Breeding, Phenotyping, Distribution anhiving of Vertebrate Mod Arcdels
Biomedical Imaging Facilities
Embryos cryopreservation, sperm cryoconservation, organs, biological material (RNA, DNA, proteins…).
Genetic engineering: creation of new transgenic organism models.
Distribution of genetically modified mouse strains.
Husbandry and breeding of several organisms according the center: mouse, rat, other rodent, non human primate, xenope, zebra-fish.
Monitoring the health status of a mouse line (quarantine, decontamination, health/sanitary control), mouse husbandry, mouse breeding, genetic background fixation, genotyping, generation of cohorts, genetic quality control of a mouse model (validation of genetic modification, level of gene expression and tissue expression).
Creation of genetically modified mouse models by transgenesis and targeted mutagenesis, production of ES cells, screening and validation of recombinated ES cells.
CELPHEDIA partners offer guidance in the choice of the organism model and a wilde range of courses and workshops for the use of the model.
Clinical phenotyping in vivo in the field of anatomic-pathology, behavior, cognition and sensory systems, cardiovascular system, neuromuscular system, nutrition and metabolism, renal function, pulmonary,embryology and reproduction, immunology, inflammation, allergy, infectiology, oncology, clinical chemistry and hematology), in vivo imaging (photonic, 2D and 3D radiologic, isotopic imaging), in vitro and in situ immunophenotyping ( Innate and adaptive immune sytem under normal or confined condition),.
Distribution of wild-type organisms, modified strains, embryos, eggs (fish, xenope), biological material to users.
In vivo model development (mouse) tools; animal testing training (mouse, pig, rabbit); stereotaxy; xenografs; BrainSight type neuro-navigation device.
Closed circuit aquarium (xenope, fish).
Housing and breeding rooms for mouse and rat under various health status (SPF, SOPF, biosafety containment from A2 to A3), in various devices (opened cages, ventilated racks) and with controlled environnement (temperature, light, photoperiod, food quantity).
For non-invasive exploration through: molecular, microscopic, mesoscopic, macroscopic imaging tools.
Facs analysers; cell sorter; cell separator; Luminex; mass cytometer; polychromatic flow cytometry devices; BSL3 live imaging (confocal and biphotonic imaging).
Material for functional exploration (HPLC, OLYMPUS AU-400 automated laboratory work station, luminex, facs analyser, metabolic cages, comprehensive indirect calorimetry system, exercise treadmill, NMR body composition, echocardiographs, blood pressure measurment device, plethysmograph, telemetry devices); material for testing cognitive functions and behavior (openfield, Y- maze, Morris water-maze, slit lamp imaging, ERG, clic box); histopathology platform.
For non-invasive exploration by molecular imaging, isotopic imaging, and radiology imaging: bioluminiscence, fluorescence, scintigraphy, positron emission tomography, and tomography opto-acoustic equipment.
Conventional housing; A2 containment housing; external dedicated areas.
Genetically modified strains creation: DNA and ES cells microinjection; microinjection devices for ovocytes; robotic (Tecan) workstations for DNA; microfluidics electrophoresis robots (Caliper); current molecular biology devices (PCR, qPCR, blots, etc...).