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French National Infrastructure for Mouse Phenogenomics (PHENOMIN)
Identification
Hosting Legal Entity
French National Infrastructure for Mouse Phenogenomics
Location
1 Rue Laurent Fries, Illkirch, PO: 67404, Strasbourg (Alsace) (France)
Structure
Type Of RI
Distributed
Coordinating Country
France
Status
Status
Current Status:
Operational since 2011
Scientific Description
PHENOMIN is a French national infrastructure for mouse phenogenomics. It is founded by 3 major national nodes: the Institut Clinique de la Souris (ICS, Illkirch), the Transgenesis and Archiving of Animal Models (TAAM, Orléans, Villejuif) and the Centre for Immunophenomics (CIPHE, Marseille) that are devoted to serving the community for the usage of mouse models. PHENOMIN constitutes a unique distributed resource for the creation, care, phenotyping, distribution and archiving of mouse models for academics and private corporations.

RI Keywords
Archiving and distribution, Genetic engineering, Zootechnics, Mouse, Phenotyping, Animal welfare
Classifications
RI Category
Animal facilities
Cell Culture Facilities
Biomedical Imaging Facilities
Repositories
Scientific Domain
Biological and Medical Sciences
ESFRI Domain
Health and Food
Services
Phenotyping

Clinical phenotyping in vivo in the following fields: anatomic-pathology, behavior, cognition, sensory systems, cardiovascular system, neuromuscular system, nutrition and metabolism, renal function, pulmonary function, embryology and reproduction, immunology, inflammation, allergy, infectiology, oncology, clinical chemistry and hematology); in vivo imaging (photonic, 2D and 3D radiologic, isotopic imaging); in vitro and in situ immunophenotyping (of innate and adaptive immune sytem under normal or confined condition).

Genetic Engineering

Creation of genetically modified mouse models by transgenesis and targeted mutagenesis, production of ES cells, screening and validation of recombinated ES cells.

Distribution

Distribution of genetically modified mouse strains.

Archiving

Mouse embryo and spermatozoa cryopreservation.

Training

A panel of courses and workshops in mouse phenogenomics.

Zootechnics

Monitoring the health status of a mouse line (quarantine, decontamination, health/sanitary control), mouse husbandry, mouse breeding, genetic background fixation, genotyping, generation of cohorts, genetic quality control of a mouse model (validation of genetic modification, level of gene expression and tissue expression).

Equipment
Rodent Breeding Facilities

Housing and breeding rooms for mouse and rat under various health statuses (SPF, SOPF, biosafety containment from A2 to A3); various housing and breeding devices: open cages, ventilated racks; controlled environnement (controlling for temperature, light, photoperiod, food quantity, etc.).

Platform for Small Animal Imaging

For non-invasive exploration by molecular imaging, isotopic imaging, and radiology imaging through: bioluminiscence, fluorescence, scintigraphy, positron emission tomography, tomography opto-acoustic.

Mutagenesis Platform

For the creation of genetically modified strains: DNA and ES cell microinjection devices; ovocyte microinjection devices; robotic (Tecan) workstations for DNA; microfluidics electrophoresis robots (Caliper), current molecular biology devices (PCR, qPCR, blots, etc.).

Phenotyping Platform

Materials for functional exploration: HPLC, OLYMPUS AU-400 automated laboratory work station, Luminex, FACS analyser, metabolic cages, comprehensive indirect calorimetry system, exercise treadmill, NMR body composition, echocardiographs, blood pressure measurment device, plethysmograph, telemetry devices; materials for cognitive function and behavior exploration: openfield, Y- maze, Morris water-maze, slit lamp imaging, ERG, clic box; histopathology platform.

Immunophenotyping Platform

FACS analysers; cell sorter; cell separator; Luminex; mass cytometer; polychromatic flow cytometry devices; BSL3 live imaging (confocal and biphotonic imaging).

Microsurgery Facilities

For in vivo animal model development (mouse): stereotaxis, xenograft BrainSight type neuro-navigation device; training for animal testing (mouse, pig, rabbit).

Date of last update: 28/02/2017
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