Helmholtz Association
|
|
Address:
|
Germany
|
Leibniz Association
|
|
Address:
|
Germany
|
Biological and Medical Sciences
The service offered to projects contains the detailed information about the screening technologies established in the Unit to potential users, including the requirements of the HTS-testsystems with the appropriate assay conditions. In an application form the project responsibilities (PI, contact details), rules in accordance to Genetic Engineering Act as well as conditions for the support of the project are defined. In the first phase the visiting scientists transfer their tests to the Screening Unit Berlin-Buch. This phase will be finished if the quality of the assay meets the requirements of the HTS acceptance test. In the second phase the HTS compound screening and the genome-wide RNA interference with oligonucleotides is conducted in the laboratory of the Screening Unit. By means of state-of-the-art automation 35.000 single experiments per day can be carried out in 384 well plate format with complex robotic systems. Several routine assay controls are established for monitoring assay robustness and quality during the screen as well as in the final analysis and identification of bioactive molecules (small compounds and RNA-oligonucleotides). A maximum of 352 compounds will be selected and evaluated in dose dependent measurements (IC50-determination). Additionally, the integrity and purity of each hit are determined via LC-MS quality controls. A limited capacity of medicinal chemistry support is available for selected projects in order to re-synthesize the initial hit or a focussed library. Until 2015 the Screening Unit Berlin-Buch has supported over 200 Screening projects and aims to opitmize its workflows even further.
Xcelligence.
4 incubators.
Caliper LabChip3000.
5 Tecan Safire Reader (spectroscopy).
Biacore T200
RNA interference.
4 Tecan liquid handling stations.
For cell-based tests.
Agilent TOF.
Liconic Kiwi Store; REMP SSS Store.