Czech Academy of Sciences
Gross pathology and tissue collection, tissue fixation, processing and embedding, sectioning, histological staining, immunohistochemistry, in situ hybridization, tissue arrays, digital slide scanning.
Cryopreservation and archiving of rodent embryos and sperm; import/export of live animals or cryopreserved material; reanimation of cryopreserved material by IVF or surgical implantation National node of the European Mutant Mouse Archive.
Services include imaging (microcomputed tomography and optical projection tomography), immunohistochemistry and in situ hybridization (section and whole-mount), alcian blue/alizarin red staining of cleared embryonic skeletons, late gestation vascular anatomy (india ink), beta-galactosidase staining.
Generation of custom genetically modified mouse and rat models by targeted mutagenesis and transgenesis (TALEN and Cas9/CRISPR, ES cell derivation, culture, electroporation and screening, pronuclear microinjection and blastocyst injections).
Mouse and rat husbandry and breeding (SPF facility, compliant with FELASA recommendations).
Systematic standardized phenotyping in accordance with EMPReSS SOPs: assessment and measurement of behavior, metabolism, gross anatomy and body composition, hematology, clinical blood chemistry and urinalysis, immunology, cardiovascular and lung function.
Equipment for echocardiography, electrocardiography, dual energy X-ray absoptiometry (DEXA), auditory brainstem response, fluorescence-activated cell sorting (FACS), indirect calorimetry, plethysmography, blood chemistry analysis, gait analysis, eye morphometry and behavioral analysis (open-field, water maze, elevated plus maze, Y-maze, startle reflex etc.).
Optical projection tomography, micro-computed tomography, dissecting microscopes etc.
Automated vacuum tissue processor, tissue embedding center, automated and rotary microtomes, cryostats, Ventana Symphony and Ventana BenchMark Special Stains.
micro-manipulating microscope systems, incubators, preparation and surgery microscopes, micropipette puller, microforge, laminar-flow benches, incubators, etc.
Breeding cages with racks (IVC and open), autoclaves, sterilization chambers, washing areas etc.
controlled rate freezer, equipment for both ICSI and standard IVF, liquid nitrogen storage tanks with automated filling station etc.