Czech Academy of Sciences
Cryopreservation and archiving of rodent embryos and sperm; import/export of live animals or cryopreserved material; reanimation of cryopreserved material by IVF or surgical implantation National node of the European Mutant Mouse Archive.
Services include imaging (microcomputed tomography and optical projection tomography), immunohistochemistry and in situ hybridization (section and whole-mount), alcian blue/alizarin red staining of cleared embryonic skeletons, late gestation vascular anatomy (india ink), beta-galactosidase staining.
Systematic standardized phenotyping in accordance with EMPReSS SOPs: assessment and measurement of behavior, metabolism, gross anatomy and body composition, hematology, clinical blood chemistry and urinalysis, immunology, cardiovascular and lung function.
Generation of custom genetically modified mouse and rat models by targeted mutagenesis and transgenesis (TALEN and Cas9/CRISPR, ES cell derivation, culture, electroporation and screening, pronuclear microinjection and blastocyst injections).
Mouse and rat husbandry and breeding (SPF facility, compliant with FELASA recommendations).
Gross pathology and tissue collection, tissue fixation, processing and embedding, sectioning, histological staining, immunohistochemistry, in situ hybridization, tissue arrays, digital slide scanning.
controlled rate freezer, equipment for both ICSI and standard IVF, liquid nitrogen storage tanks with automated filling station etc.
micro-manipulating microscope systems, incubators, preparation and surgery microscopes, micropipette puller, microforge, laminar-flow benches, incubators, etc.
Optical projection tomography, micro-computed tomography, dissecting microscopes etc.
Automated vacuum tissue processor, tissue embedding center, automated and rotary microtomes, cryostats, Ventana Symphony and Ventana BenchMark Special Stains.
Equipment for echocardiography, electrocardiography, dual energy X-ray absoptiometry (DEXA), auditory brainstem response, fluorescence-activated cell sorting (FACS), indirect calorimetry, plethysmography, blood chemistry analysis, gait analysis, eye morphometry and behavioral analysis (open-field, water maze, elevated plus maze, Y-maze, startle reflex etc.).
Breeding cages with racks (IVC and open), autoclaves, sterilization chambers, washing areas etc.