Proteomics aims to characterize proteomes in-depth with a view to functional proteomics and clinical applications. This leads to new challenges, including how to determine variations within the proteome as a result of various stimuli or different cellular states, detect low abundance proteins important for biology or health, study the dynamics of protein complexes, analyze post-translational modifications playing a key role in protein function. In this highly challenging context, France has created the Proteomics French Infrastructure (ProFI) in 2012 for a 7 years funding (15 M€). It is a shared infrastructure which groups together the three most renowned French proteomics platforms: the “Exploring the Dynamics of Proteomes” Laboratory (CEA, INSERM and Grenoble Alpes University), the “Laboratoire de Spectrométrie de Masse Bio-Organique” (CNRS and Strasbourg University) and the “Proteomics and Mass Spectrometry of Biomolecules” research group (CNRS and Toulouse Paul Sabatier University). The objectives of ProFI are twofold: (1) R&D activities in quantitative proteomics and in bioinformatics; (2) make the services offered by ProFI widely available to the scientific community and industrial actors by setting up a highly technical environment compatible with the production and treatment of high quality data at very high throughput and in a context of quality assurance certification.