LGDB uses phenol extraction method with specific modifications for fresh of frozen blood extraction, isolated frozen buffy coat DNA extraction. The procedure for DNA isolation has been tested in quality assurance scheme for Biospecimen Proficiency Testing 2017/2018. year cycle at IBBL with highest possible evaluation mark. Laboratory provides DNA extraction from various biological material as a service.
LGDB is equipped with liquid handling robots (for DNA dilution aliquoting, plate preparation), low temperature freezers, liquid nitrogen containers, for storage of biological materials. The storage of biological materials is available as a service.
Targeted genotyping using RT-systems, Sanger sequencing or Next-Generation Sequencing using IonTorrent PGM and IonProton platforms. Services include experimental design, library preparation, sequencing and bioinformatics analysis. Most frequently used analysis includes TaqMan genotyping, Sanger sequencing, AmpliSeq targeted gene sequencing, exome sequencing, 16S RNA sequencing, transcriptome sequencing, metagenome sequencing, whole small genome analysis.
LGDB stores collection of biological material of over 32 000 donors from Latvian population, this includes population and disease based cohorts. The standard set of biosamples stored in the LGDB consists of DNA, plasma, serum and white blood cells, in some cohorts complemented by cancer biopsies, microbiome and urine samples. The data stored at LGDB includes core health heredity questionnaire, diagnosis (ICD10), biochemistry test results and genotypes. The additional data about donors could be obtained from state health system on project based purposes. The samples and data can be accessed for research. For detailed information about biobank design, available samples and data see: Rovite et al. J Epidemiol. 2018.